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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	triple negative breast cancer
ICD-0-3	C50
Methods	qRT-PCR, Fluorescence in situ hybridization, Wound healing and transwell assays, Immunoblot analysis, RIP, Biotinylated RNA pull-down assay, Luciferase reporter assay, ChIP, Immunohistochemistry (IHC), Animal studies
Sample	TNBC tissues, normal breast epithelial cell line (MCF10A) and breast cancer cell lines (MCF-7, T47D, SK-BR-3, MDA-MB-231, MDA-MB-468 and BT549)
Expression Pattern	up-regulated
Function Description	CircANKS1B was significantly up-regulated in triple-negative breast cancer (TNBC) compared with non-TNBC tissues and cell lines. Increased circANKS1B expression was closely associated with lymph node metastasis and advanced clinical stage and served as an independent risk factor for overall survival of breast cancer patients. Functional studies revealed that circANKS1B promoted breast cancer invasion and metastasis both in vitro and in vivo by inducing epithelial-to-mesenchymal transition (EMT), while had no effect on breast cancer growth. Mechanistically, circANKS1B abundantly sponged miR-148a-3p and miR-152-3p to increase the expression of transcription factor USF1, which could transcriptionally up-regulate TGF-?1 expression, resulting in activating TGF-?1/Smad signaling to promote EMT. Moreover, we found that circANKS1B biogenesis in breast cancer was promoted by splicing factor ESRP1, whose expression was also regulated by USF1.
Pubmed ID	30454010
Year	2018
Title	The pro-metastasis effect of circANKS1B in breast cancer

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