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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	osteosarcoma
ICD-0-3	NA
Methods	qRT-PCR , western blot , luciferase reporter assay , RIP , RNA pull down , cell proliferation assays, cell apoptosis detection , transwell assay
Sample	human osteosarcoma cell lines (143B, HOS, MG-63, Saos-2 and U-2 OS), and normal human osteoblast hFOB 1.19
Expression Pattern	up-regulated
Function Description	Circ_ORC2 was generally up-regulated in various osteosarcoma cell lines, and mainly distributed in the cytoplasm. Circ_ORC2 had the binding site of miR-19a, and its expression was positively correlated with miR-19a expression.Circ_ORC2 could bind to Ago2 protein.Circ_ORC2 could directly interact with miR-19a, and miR-19a could bind to circ_ORC2. After circ_ORC2 knockdown, miR-19a expression was down-regulated, but the downstream target gene PTEN expression was up-regulated, and the phosphorylation level of Akt was reduced, which indicated that circ_ORC2 enhanced the inhibition of miR-19a on PTEN expression by combining miR-19a. After circ_ORC2 knockdown, cell proliferation and invasion decreased, while the apoptosis level increased. When co-transfected with circ_ORC2 siRNA and miR-19a mimics or PTEN siRNA, the above cell biological behaviors did not change significantly. Therefore, circ_ORC2 binds with miR-19a and enhances its expression, thereby inhibiting downstream PTEN expression and activating Akt pathway to promote osteosarcoma cell growth and invasion.
Pubmed ID	31103262
Year	2019
Title	Circ_ORC2 Enhances the Regulatory Effect of miR-19a on Its Target Gene PTEN to Affect Osteosarcoma Cell Growth

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