Detail

Basic Information

LncRNA/CircRNA Name	circNR3C1
Synonyms
Region
Ensemble
Refseq

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	NA	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	bladder cancer
ICD-0-3	C67
Methods	qRT-PCR , western blot , luciferase reporter assay , northern blotting , RNase R treatment , PCR amplification and sanger sequencing , pull-down assay , CCK-8 assay , 5-Ethynyl-20-deoxyuridine (EdU) assay , colony formation assay , soft agar colony formation assay , cell cycle assay , tumor xenografts experiments , immunohistochemistry
Sample	bladder cancer and paired adjacent normal bladder tissues ,human metastatic bladder cancer cell line T24T ,human bladder cancer cell lines(EJ, UMUC3, J82, 5637) and human immortalized uroepithelium cell line (SV-HUC-1)
Expression Pattern	down-regulated
Function Description	In this study, we discovered that a circular RNA derived from NR3C1 (circNR3C1) was downregulated in bladder cancer tissues and cell lines. Functionally, we found that overexpression of circNR3C1 could significantly inhibit cell cycle progression and proliferation of bladder cancer cells in vitro, as well as suppress tumor growth in vivo. Mechanistically, circNR3C1 possessed four targeting sites of miR-27a-3p and could effectively sponge miR-27a-3p to suppress the expression of cyclin D1. Furthermore, miR-27a-3p functioned as an oncogene through interacting with 5'UTR of cyclin D1 to enhance its expression, which led to promote cell cycle progression and proliferation in bladder cancer cells.
Pubmed ID	31255724
Year	2019
Title	CircNR3C1 Inhibits Proliferation of Bladder Cancer Cells by Sponging miR-27a-3p and Downregulating Cyclin D1 Expression

External Links

Links for circNR3C1	GenBank HGNC NONCODE
Links for bladder cancer	OMIM COSMIC