Basic Information
| LncRNA/CircRNA Name | ZEB2-AS1 |
| Synonyms | NA |
| Region | GRCh38_2:144518097-144521477 |
| Ensemble | ENSG00000238057 |
| Refseq | NR_040248 |
Classification Information
| Regulatory Mechanism | Biological Function | Clinical Application | |||
|---|---|---|---|---|---|
| TF | Immune | Survival | |||
| Enhancer | Apoptosis | Drug | |||
| Variant | Cell Growth | Circulating | |||
| MiRNA | EMT | Metastasis | |||
| Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
| Cancer Name | gastric cancer |
| ICD-0-3 | C16 |
| Methods | qPCR, Western blot, Luciferase reporter assay, in vitro knockdown, etc. |
| Sample | gastric cancer tissues, gastric cancer cell lines (MKN-28, BGC-823, and SGC-7901) and normal gastric cell line (GES-1) |
| Expression Pattern | up-regulated |
| Function Description | Knockdown of ZEB2-AS1 suppressed gastric cancer cell proliferation, growth and invasion, and also suppressed in vivo tumor growth in the nude mice. Overexpression of ZEB2- AS1 potentiated gastric cancer cell proliferation, growth and invasion. Bioinformatics analysis and luciferase reporter assay showed that miR-143-5p was a direct target of ZEB2-AS1 and was negatively regulated by ZEB2-AS1. Furthermore, hypoxia-inducible factor-1? (HIF-1?) was found to be a target of miR-143-5p and was negatively regulated by miR-143-5p. The rescue in vitro assays showed that the effects of ZEB2-AS1 overexpression on gastric cancer cell proliferation, growth and invasion was mediated via miR-143-5p/HIF-1?. ZEB2-AS1 and HIF-1? was upregulated in gastric cancer tissues, while miR-143-5p was down-regulated; and ZEB2-AS1 expression level was inversely correlated with miR-143-5p expression level, and positively correlated with HIF-1? mRNA expression level; while miR-143-5p expression level was inversely correlated with HIF-1? expression level. High ZEB2-AS1 expression level was correlated with poor differentiation, lymph node metastasis and distant metastasis. Conclusion: Collectively, our results indicated that ZEB2-AS1 was up-regulated in gastric cancer tissues and cells and promoted cell proliferation and metastasis through miR-143-5p/ HIF-1? pathway, which may provide a promising target for treatment of gastric cancer. |
| Pubmed ID | 30705594 |
| Year | 2019 |
| Title | The lncRNA ZEB2-AS1 is upregulated in gastric cancer and affects cell proliferation and invasion via miR-143-5p/HIF-1? axis. |
External Links
| Links for ZEB2-AS1 | GenBank HGNC NONCODE |
| Links for gastric cancer | OMIM COSMIC |