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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	osteosarcoma
ICD-0-3	NA
Methods	Western blot, Luciferase reporter assay, etc.
Sample	OS tissues, The MG63, U2OS, and Saos-2 human OS cell lines and the hFOB1.19 human osteoblast cell line
Expression Pattern	up-regulated
Function Description	the lncRNA SNHG1 was upregulated and miRNA 101 3p was downregulated in OS tissues and cell lines. Second, the knockdown of lncRNA SNHG1 induced cell apoptosis and maintained the cell cycle at the G0/G1 phase, which decreased the overall cell viability. Furthermore, according to a dual luciferase assay and western blot analysis, miRNA 101 3p was found to be a target of the lncRNA SNHG1 in OS, which further regulated the expression of Rho associated coiled coil containing protein kinase 1 (ROCK1). It was found that the phosphoinositide 3 kinase/ATK pathway was inactivated and that epithelial mesenchymal transition was activated in OS cell lines with overexpression of the lncRNA SNHG1. Taken together, in OS cell lines, the lncRNA SNHG1 acted as an oncogene, and miRNA 101 3p was considered a tumor suppressor. The lncRNA SNHG1 promoted OS cell proliferation, migration and invasion by downregulating the expression of miRNA 101 3p, which enhanced the expression of ROCK1.
Pubmed ID	30592267
Year	2019
Title	lncRNA SNHG1 negatively regulates miRNA-101-3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma

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