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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	breast cancer
ICD-0-3	C50
Methods	qPCR, Western blot, in vitro knockdown, etc.
Sample	breast cancer tissues, Human breast cancer cell lines T47D, MCF7, MDA-MB-231, MDA-MB-468 and SK-BR-3, breast epithelial cell line MCF10A
Expression Pattern	up-regulated
Function Description	RUSC1 AS N was upregulated in tumor tissues compared with in adjacent non cancerous counterparts. In addition, using several breast cancer cell lines, it was demonstrated that the mRNA levels of RUSC1 AS N were highest in the notably metastatic cell lines MDA MB 231 and MDA MB 468. Knockdown of RUSC1 AS N in breast cancer cells inhibited cell proliferation in the colony formation and cell proliferation assays. Furthermore, depletion of RUSC1 AS N suppressed cell metastasis, as revealed by wound healing and western blot assays. In addition, the protein levels of Wnt1 and ? catenin were significantly decreased when RUSC1 AS N was knocked down. However, Wnt signaling pathway activator Wnt agonist 1 reversed the effects of RUSC1 AS N knockdown on cell proliferation and metastasis. The present study demonstrated that lncRNA RUSC1 AS N promoted cell viability and metastasis via Wnt/? catenin signaling in human breast cancer, which may indicate novel targets for the treatment of breast cancer in clinic.
Pubmed ID	30569097
Year	2019
Title	Long noncoding RNA RUSC1-AS-N promotes cell proliferation and metastasis through Wnt/?-catenin signaling in human breast cancer

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