qPCR, Western blot, Luciferase reporter assay, in vitro knockdown etc.
LSCC and adjacent non-tumorous tissues, cells lines (TU-212, AMC-HN-8, HEK-293T)
Mechanically, we found that RP11-159K7.2 could positively regulate the expression of DNMT3A by sponging miR-206. In addition, a feedback loop was also discovered between DNMT3A and miR-206. To sum up, these findings suggest that lncRNA RP11-159K7.2 could be used as a potential biomarker for prognosis and treatment of LSCC.