qRT-PCR, Western blot, Luciferase reporter assay, in vitro knockdown
lung cancer tissues, lung cancer cell lines (A549, H226, H1299 and H446)
PVT1-5 expression was significantly increased in lung cancer tissues and cell lines.miR-126 was associated with lncRNA-PVT1-5. Furthermore, knockdown of lncRNA-PVT1-5 in cells could down-regulate the expression of SLC7A5, the target of oncogenic miR-126, resulting in the cell proliferation. Conversely, inhibiting the expression of miR-126 markedly increased the expression of SLC7A5 and alleviated cell proliferation inhibition.