Search Result

Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	gastric cancer
ICD-0-3	C16
Methods	qPCR, Western blot, Luciferase reporter assay, in vitro knockdown, RIP
Sample	gastric cancer tissues, cell lines (MKN-45, MKN-74, NCI-N87 and AGS)
Expression Pattern	up-regulated
Function Description	Real-time quantitative RT-PCR (qRT-PCR) revealed higher pancEts-1 levels in cultured gastric cancer cells, when compared to those in normal gastric mucosa. Ets-1 promoter-associated noncoding RNA (pancEts-1) as a novel lncRNA associated with the gastric cancer progression via mining of publicly available datasets and rapid amplification of cDNA ends. RNA pull-down, RNA immunoprecipitation, in vitro binding, and RNA electrophoretic mobility shift assays indicated the binding of pancEts-1 to non-POU domain containing octamer binding (NONO) protein. Mechanistically, pancEts-1 facilitated the physical interaction between NONO and Ets related gene (ERG), resulting in increased ERG transactivation and transcription of Ets-1 associated with gastric cancer progression. In addition, pancEts-1 facilitated the growth and aggressiveness of gastric cancer cells via interacting with NONO. In gastric cancer tissues, pancEts-1, NONO, and ERG were upregulated and significantly correlated with Ets-1 levels. High levels of pancEts-1, NONO, ERG, or Ets-1 were respectively associated with poor survival of gastric cancer patients, whereas simultaneous expression of all of them (HR = 3.012, P = 0.105) was not an independent prognostic factor for predicting clinical outcome.
Pubmed ID	29773901
Year	2018
Title	Ets-1 Promoter-Associated Noncoding RNA Regulates the NONO/ERG/Ets-1 Axis to Drive Gastric Cancer Progression

External Links