Basic Information
| LncRNA/CircRNA Name | OIP5-AS1 |
| Synonyms | NA |
| Region | GRCh38_15:41283990-41309737 |
| Ensemble | ENSG00000247556 |
| Refseq | NR_026757 |
Classification Information
| Regulatory Mechanism | Biological Function | Clinical Application | |||
|---|---|---|---|---|---|
| TF | Immune | Survival | |||
| Enhancer | Apoptosis | apoptosis | Drug | Cisplatin | |
| Variant | Cell Growth | Circulating | |||
| MiRNA | EMT | Metastasis | |||
| Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
| Cancer Name | osteosarcoma |
| ICD-0-3 | NA |
| Methods | qPCR, Western blot, Luciferase reporter assay, in vitro knockdown, etc. |
| Sample | human osteosarcoma cell lines, MG63 and SaOS2 |
| Expression Pattern | up-regulated |
| Function Description | OIP5 AS1 was significantly upregulated in cisplatin resistant (CR) osteosarcoma cells MG63 CR and SaOS2 CR compared with the corresponding parental cells. OIP5 AS1 silencing suppressed cell growth in vitro and in vivo, and promoted apoptosis of MG63 CR and SaOS2 CR cells, indicating that knockdown of OIP5 AS1 significantly decreased cisplatin resistance in MG63 CR and SaOS2 CR cells. This conclusion was supported by the decreased expression of the drug resistance related factors multidrug resistance associated protein 1 (MRP1) and P glycoprotein (P gp) upon OIP5 AS1 silencing. In addition, OIP5 AS1 downregulation suppressed the PI3K/ AKT/mTOR signaling pathway. Importantly, we demonstrated that OIP5 AS1 functions as a competing endogenous RNA of miR 340 5p and regulates the expression of lysophosphatidic acid acyltransferase (LPAAT?), which is a target of miR 340 5p. Moreover, downregulation of miR 340 5p partly reversed the inhibitory effect of OIP5 AS1 knockdown on the PI3K/AKT/mTOR pathway and therefore counteracted cisplatin resistance in MG63 CR and SaOS2 CR cells. In conclusion, OIP5 AS1 causes cisplatin resistance in osteosarcoma through inducing the LPAAT?/PI3K/AKT/mTOR signaling pathway by sponging the miR 340 5p. Our results contribute to a better understanding of the function and mechanism of OIP5 AS1 in osteosarcoma cisplatin resistance. |
| Pubmed ID | 30548308 |
| Year | 2018 |
| Title | Long noncoding RNA OIP5-AS1 causes cisplatin resistance in osteosarcoma through inducing the LPAAT?/PI3K/AKT/mTOR signaling pathway by sponging the miR-340-5p |
External Links
| Links for OIP5-AS1 | GenBank HGNC NONCODE |
| Links for osteosarcoma | OMIM COSMIC |