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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	colorectal cancer
ICD-0-3	C19.9
Methods	qPCR, Western blot, Luciferase reporter assay, in vitro knockdown, RIP
Sample	Human CRC cell lines HCT116, SW480, LoVo, RKO and C2BBel-Caco-2, the normal colorectal epithelial cell line FHC , CRC tissues and matched adjacent normal tissues
Expression Pattern	up-regulated
Function Description	an antisense lncRNA of NR4A1 that was upregulated in CRC tissues and cells with rapid amplification of cDNA ends, and designated it as NR4A1AS. Spearman correlation analysis showed that NR4A1AS was positively correlated with NR4A1 mRNA levels in 37 CRC tissues. Mechanistically, NR4A1AS stabilized NR4A1 mRNA by forming RNA-RNA complexes via partial base-pairing and upregulated NR4A1 expression in CRC cells. RNA immunoprecipitation assays revealed that knockdown of NR4A1AS expression by siRNA enhanced UPF1 recruitment to NR4A1 mRNA, thereby decreasing NR4A1 mRNA stability. Moreover, depletion of NR4A1AS was found to mimic the effect of NR4A1 knockdown, specifically by suppressing cell proliferation, migration and invasion, and inducing apoptosis and cell cycle arrest. Accordingly, restoring NR4A1 expression ameliorated the effects of NR4A1AS knockdown on tumor growth and metastasis of CRC cells in vitro and in vivo.
Pubmed ID	31253658
Year	2019
Title	A novel lncRNA NR4A1AS upregulates orphan nuclear receptor NR4A1 expression by blocking UPF1-mediated mRNA destabilization in colorectal cancer

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