Microarray, QRT-PCR, Western blot, Luciferase reporter assay, in vitro knockdown
PTC tissues, Thyroid papillary cell line NPA87 (BNCC338081), TPC-1(BNCC337912) and KAT-5 (BNCC338083)
LncRNA NEAT1 was highly expressed in PTC tissues and cell lines and could deteriorate PTC by promoting proliferation, invasion, and migration accompanied by less apoptosis. Besides, miR-129-5p/lncRNA NEAT1 were found to negatively correlate with each other by direct target relationship and their combination suppressed the progression of PTC. KLK7,a highly expressed downstream protein in PTC tissues,could be directly regulated by miR-129-5p in a negative way. KLK7 accelerated the deterioration of PTC in vitro experiments which could be reversed by sh-lnc RNA NEAT1 and miR-129-5p mimics.In vivo experiments, silence of lncRNA NEAT1 restrain tumor growth in weight and volume.