Search Result

Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	ovarian cancer
ICD-0-3	C56.9
Methods	qPCR, Western blot, Luciferase reporter assay, in vitro knockdown
Sample	ovarian cancer tissues, cell lines (IOSE80, ES2 and SKOV3)
Expression Pattern	up-regulated
Function Description	NEAT1 was upregulated in OC tissue specimens and cell lines. Highly-expressed NEAT1 was closely correlated with a shorter survival rate, a poor differentiated degree, a bigger tumor size, an advanced FIGO stage and significant peritoneal metastasis. In addition, we revealed that depression of NEAT1 inhibited OC cells' metastasis and the expression of Rho associated coiled-coil containing protein kinase 1 (ROCK1), which is a metastasis-related gene. Using online predictive software and a series of luciferase assays, we demonstrated that both NEAT1 and ROCK1 were the targets of microRNA-382-3p (miR-382-3p) and share similar microRNA responding elements (MRE). Furthermore, we illustrated that NEAT1 and miR-382-3p inhibited each other in a reciprocal manner. Finally, through antisense experiments we demonstrated that NEAT1 promoted ROCK1-mediated metastasis by functioning as a ceRNA of miR-382-3p.
Pubmed ID	29790629
Year	2018
Title	Long Non-Coding RNA NEAT1 Promoted Ovarian Cancer Cells' Metastasis Through Regulation of miR-382-3p/ROCK1 Axial

External Links