microarray, qPCR, RNAi, Western blot, Luciferase reporter assay etc.
cell lines (HNE2, HNE1, CNE2)
LOC401317 was the most significantly upregulated lncRNA. Further studies indicated that LOC401317 is diretcly regulated by p53 and that etcopic expression of LOC401317 inhibits HNE2 cell proliferation in vitro and in vivo by inducing cell cycle arrest and apoptosis. LOC401317 inhibited cell cycle progression by increasing p21 expression and decreasing cyclin D1 and cyclin E1 expression and promoted apoptosis through the induction of poly(ADP-ribose) polymerase and caspase-3 cleavage.