The normal human pancreatic ductal cell line (hTERT-HPNE), Human pancreatic cancer cell lines (PANC-1, MiaPaCa-2, BxPC-3 and AsPC-1) and 293T cells, PC and non-tumor tissues
lnc-PCTST expression is down-regulated in pancreatic cancer tissues. Also, lnc-PCTST has shown to be negatively correlated with transforming acidic coiled-coil 3 (TACC-3) expression.lnc-PCTST overexpression in pancreatic cancer cell lines inhibited cell proliferation and invasion in vitro, and tumorigenesis in vivo, but did not altered cell migration. Additionally, TACC-3 knockdown simulated the inhibiting effect of lnc-PCTST overexpression on pancreatic cancer cell lines, and the impaired proliferation, invasion effect and E-cadherin, vimentin expression on lnc-PCTST over-expressed cell lines can be rescued by overexpressed TACC-3. Significantly, the expression of lnc-PCTST was closely associated with its genomic neighboring gene TACC-3 and inhibited its promoter activity.