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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	non small cell lung cancer
ICD-0-3	C34
Methods	qPCR, Western blot, Luciferase reporter assay, in vitro knockdown, RIP, etc.
Sample	lung cancer tissue, Human NSCLC cell lines A549 and PC9
Expression Pattern	down-regulated
Function Description	the deregulation of lincRNA-p21 in NSCLC tumor tissues in comparison to adjacent healthy tissues was examined using reverse transcription-quantitative polymerase chain reaction. Furthermore, the effect of lincRNA-p21 overexpression and knockdown on different NSCLC cell lines was further investigated in vitro. The association between lincRNA-p21 expression and microRNA (miR)-17-5p level in NSCLC tumor cells was also investigated to clarify the underlying mechanism. The influence of miR 17 5p on different NSCLC cell lines A549 and PC9 were also examined in vitro using miR-17-5p mimics and inhibitors. Bioinformatics and luciferase assays were conducted to verify the direct binding sites on lincRNA-p21 for miR-17-5p. The results demonstrated that there was a significant low expression of lincRNA p21 in NSCLC tumor tissues, and lincRNA-p21 effectively inhibited the progression of lung cancer cells by suppressing cell proliferation and migration and promoting cell apoptosis. An evident negative association between lincRNA-p21 and miR-17-5p expression was observed, and the inhibitory effect of overexpressed lincRNA-p21 on lung cancer cells was counteracted by miR-17-5p. Bioinformatics and luciferase reporter analysis results confirmed that miR 17 5p is a direct target for lincRNA-p21.
Pubmed ID	30535441
Year	2019
Title	LincRNA-p21 inhibits the progression of non-small cell lung cancer via targeting miR-17-5p

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