Basic Information
| LncRNA/CircRNA Name | KCNQ1OT1 |
| Synonyms | NA |
| Region | GRCh38_11:2608328-2699994 |
| Ensemble | ENSG00000269821 |
| Refseq | NR_002728 |
Classification Information
| Regulatory Mechanism | Biological Function | Clinical Application | |||
|---|---|---|---|---|---|
| TF | Immune | Survival | |||
| Enhancer | Apoptosis | apoptosis | Drug | methotrexate (MTX) | |
| Variant | Cell Growth | Circulating | |||
| MiRNA | EMT | Metastasis | |||
| Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
| Cancer Name | colorectal cancer |
| ICD-0-3 | C19.9 |
| Methods | qRT-PCR, Microarray analysis etc. |
| Sample | Colorectal cancer tumour tissues (n = 20) and MTX-resistant CRC tumour tissues (n = 20), The human CRC cell line HT29 and Caco2 cells |
| Expression Pattern | up-regulated |
| Function Description | LncRNA KCNQ1OT1 and PPP1R1B mRNA were overexpressed in MTX-resistant CRC tumour cells. KCNQ1OT1 functioned as a sponge of miR-760, which targeted PPP1R1B. Knockdown of KCNQ1OT1 enhanced chemosensitivity towards MTX through the sponging of miR-760. MiR-760 expressed at low levels targeted PPP1R1B in the activated cAMP signalling pathway under MTX treatment. Knockdown of KCNQ1OT1 dampened the proliferation of MTX-resistant (HT29/MTX) cells by regulating the miR-760/PPP1R1B axis, which also induced cell cycle arrest together with apoptosis. KCNQ1OT1 regulated the expression of PPP1R1B and the downstream genes CREB and CBP in the cAMP signalling pathway. |
| Pubmed ID | 30997746 |
| Year | 2019 |
| Title | LncRNA KCNQ1OT1 Enhanced the Methotrexate Resistance of Colorectal Cancer Cells by Regulating miR-760/PPP1R1B via the cAMP Signalling Pathway |
External Links
| Links for KCNQ1OT1 | GenBank HGNC NONCODE |
| Links for colorectal cancer | OMIM COSMIC |