Basic Information
LncRNA/CircRNA Name | HOTAIR |
Synonyms | HOTAIR, HOXAS, HOXC-AS4, HOXC11-AS1, NCRNA00072 |
Region | GRCh38_12:53962308-53974956 |
Ensemble | ENSG00000228630 |
Refseq | NR_003716 |
Classification Information
Regulatory Mechanism | Biological Function | Clinical Application | |||
---|---|---|---|---|---|
TF | Immune | Survival | |||
Enhancer | Apoptosis | Drug | |||
Variant | Cell Growth | Circulating | |||
MiRNA | EMT | Metastasis | |||
Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
Cancer Name | colon cancer |
ICD-0-3 | C18 |
Methods | qPCR, RNAi, Western blot, Flow cytometry assay etc. |
Sample | colon cancer tissues, cell line (HCT116) |
Expression Pattern | up-regulated |
Function Description | In 80 clinical colon cancer tissues, HOTAIR and miR-125a-5p levels were higher than adjacent tissues, whereas caspase 2 was lower. RNA sequencing detected that miR-125a-5p was decreased after HOTAIR knockdown and miR-125a-5p mimics could rescue the apoptosis induced by HOTAIR deficiency. In order to find out whether HOTAIR is essential for cancer cell survival, we transfected a specific siRNA targeting HOTAIR (siHOT1) or scrambled siRNA (siNC) into HCT116, HeLa, HepG2, A549 and DU145 cells. Each siRNA depleted HOTAIR and led to concomitant apoptosis. |
Pubmed ID | 26962687 |
Year | 2016 |
Title | MiR-125a-5p decreases after long non-coding RNA HOTAIR knockdown to promote cancer cell apoptosis by releasing caspase 2. |
External Links
Links for HOTAIR | GenBank HGNC NONCODE |
Links for colon cancer | OMIM COSMIC |