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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	hepatocellular carcinoma
ICD-0-3	C22.0
Methods	qPCR, Western blot, Luciferase reporter assay
Sample	hepatocellular carcinoma tissues, cell lines (Huh7, Hep3B, HepG2, MHCC97H and L-02)
Expression Pattern	up-regulated
Function Description	HOTAIR was overexpressed in HCC tissues and cell lines. Overexpressed HOTAIR promoted invasion and migration of HCC cells (Huh7) by enhancing epithelial-mesenchymal transition (EMT). Besides that, miR-23b-3p was predicted to be a target of HOTAIR and decreased expression of miR-23b-3p was observed in HCC tissues and cell lines. The up-regulation of HOTAIR strongly decreased the expression of miR-23b-3p. The further luciferase report confirmed the targeting reaction between HOTAIR and miR-23b-3p, suggesting that the expression of miR-23b-3p was negatively regulated by HOTAIR. Moreover, the zinc-finger E-box-binding homeobox 1 (ZEB1) protein was predicted to be a target of miR-23b-3p. The expression of ZEB1 was negatively regulated by miR-23b-3p while positively regulated by HOTAIR. Besides that, transfection with miR-23b-3p mimic counteracted the promoting effects of HOTAIR on invasion, migration and EMT of HCC cells. Our in vitro experiments suggested that HOTAIR promoted invasion and migration of HCC cells through enhancing EMT via sponging miR-23b-3p from ZEB1. Finally, the in vivo experiments indicated that HOTAIR could promote metastasis of HCC by enhancing EMT in vivo.
Pubmed ID	29778425
Year	2018
Title	LncRNA HOTAIR Contributes to the Malignancy of Hepatocellular Carcinoma by Enhancing Epithelial-Mesenchymal Transition via Sponging miR-23b-3p From ZEB1

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