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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	triple negative breast cancer
ICD-0-3	C50
Methods	qPCR, Western blot, Luciferase reporter assay
Sample	triple negative breast cancer tissues, cell lines (MCF-7, T47D, BT474, MDA-MB-231 and MDA-MB-468)
Expression Pattern	down-regulated
Function Description	Levels of the lncRNA, growth arrest-specific transcript 5 (GAS5), are decreased in TNBC tissues, and this down-regulation of GAS5 is associated with an aggressive tumor phenotype in patients, affecting clinical stage, lymph node metastasis and overall survival. Using an ectopic overexpression system in TNBC cells, we found that up-regulation of GAS5 can significantly attenuate proliferation and enhance apoptosis in TNBC cells. Through bioinformatics analysis and verification with qRT-PCR and luciferase assay, we found that GAS5 can bind to miR-196a-5p and there is a negative relationship between GAS5 and miR-196a-5p expression among TNBC patient samples. Furthermore, we demonstrated that overexpression of GAS5 can partially undermine the tumor promotion effect induced by ectopic expression of miR-196a-5p, including invasion and downstream FOXO1/PI3K/AKT signal pathway activation.
Pubmed ID	29793177
Year	2018
Title	Long Noncoding RNA GAS5 Suppresses Triple Negative Breast Cancer Progression Through Inhibition of Proliferation and Invasion by Competitively Binding miR-196a-5p

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