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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug	Tamoxifen
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	breast cancer
ICD-0-3	C50
Methods	Microarray, qPCR, Western blot, Luciferase reporter assay, in vitro knockdown, RIP
Sample	breast cancer tissues, cell lines (MCF-7 and MCF-7R)
Expression Pattern	down-regulated
Function Description	Downregulation of lncRNA GAS5 was found in MCF-7R cells. Besides, decreased expression of GAS5 was found in breast cancer tissues, which was associated with advanced TNM stage and shorter overall survival time. We further found that GAS5 overexpression enhanced cell sensitivity to tamoxifen in MCF-7R cells both in vitro and in vivo. Moreover, GAS5 increased sensitivity of breast cancer cells to tamoxifen by serving as a molecular sponge for miR-222, contributing to suppression of phosphatase and tensin homologs (PTEN) (one endogenous target of miR-222). MCF-7R celltransfected with pcDNA-GAS5 significantly increased apoptosis com-pared with cells transfected with pcDNA-NC, and apoptosis decreasedafter cells transfected with si-GAS5 compared with those transfectedwith si-NC. MTT assay showed that cell proliferation in the si-GAS5 groupwas higher than that of si-NC group at 24 h, 72 h and 96 h, while sig-nificantly lower in the pcDNA-GAS5 group compared with the pcDNA-NC group when cells were treated with 10?M tamoxifen.
Pubmed ID	29969658
Year	2018
Title	Downregulation of lncRNA GAS5 Confers Tamoxifen Resistance by Activating miR-222 in Breast Cancer

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