Basic Information
| LncRNA/CircRNA Name | AFAP1-AS1 |
| Synonyms | NA |
| Region | GRCh38_4:7754090-7778928 |
| Ensemble | ENSG00000272620 |
| Refseq | NR_026892 |
Classification Information
| Regulatory Mechanism | Biological Function | Clinical Application | |||
|---|---|---|---|---|---|
| TF | Immune | Survival | |||
| Enhancer | Apoptosis | apoptosis | Drug | Cisplatin | |
| Variant | Cell Growth | Circulating | |||
| MiRNA | EMT | Metastasis | |||
| Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
| Cancer Name | laryngeal cancer |
| ICD-0-3 | C32 |
| Methods | qPCR, Western blot, Luciferase reporter assay |
| Sample | laryngeal cancer tissues, cell lines (HEp-2/R) |
| Expression Pattern | up-regulated |
| Function Description | AFAP1-AS1 was up-regulated in laryngeal carcinoma specimens and cells, but also that stemness-associated genes were overexpressed. Silencing of AFAP1-AS1 promoted HEp-2 cell chemoresistance under cisplatin treatment. Expression of AFAP1-AS1 was increased in drug-resistant Hep-2 cells. Apoptosis assays of AFAP1-AS1-silenced HEp-2 cells treated with 8 ?mol L?? cisplatin showed that silencing AFAP1-AS1 significantly elevated cell apoptosis. Luciferase reporter and qRT-PCR assays of AFAP1-AS1 and miR-320a levels in human specimens and cell cultures indicated that AFAP1-AS1 negatively regulates miR-320a. After cloning the 3'-untranslated regions (3'-UTR) of RBPJ into a luciferase reporter, we determined that miR-320a did in fact reduce RBPJ mRNA and protein levels. Ultimately, we determined that AFAP1-AS1 increases RBPJ expression by negatively regulating miR-320a and RBPJ overexpression rescues stemness and chemoresistance inhibited by AFAP1-AS1 silencing. |
| Pubmed ID | 29971915 |
| Year | 2018 |
| Title | Long Non-Coding RNA AFAP1-AS1/miR-320a/RBPJ Axis Regulates Laryngeal Carcinoma Cell Stemness and Chemoresistance |
External Links
| Links for AFAP1-AS1 | GenBank HGNC NONCODE |
| Links for laryngeal cancer | OMIM COSMIC |