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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	laryngeal cancer
ICD-0-3	C32
Methods	qPCR, Luciferase reporter assay, in vitro knockdown, RIP, etc.
Sample	Hep-2,Hep-2R cells
Expression Pattern	up-regulated
Function Description	DGCR5 was significantly upregulated in Hep 2R cells. Inhibition of DGCR5 by LV shDGCR5 transfection restrained Hep 2R cell proliferation and sensitized cells to radiation. Reversely, overexpression of DGCR5 exhibited an opposite phenomenon in vitro. In addition, microRNA (miR) 195 was predicted as a direct downstream target of DGCR5. Dual luciferase reporter and RNA immunoprecipitation assays verified the direct interaction between them. Meanwhile, miR 195 was observed to be reduced in Hep 2R cells and miR 195 mimics repressed Hep 2 cell growth. Moreover, radiosensitivity of Hep 2R cells was greatly enhanced by overexpression of miR 195, which could be reversed by upregulation of DGCR5. Finally, in vivo experiments were used to validate that knockdown of DGCR5 suppressed laryngeal carcinoma via targeting miR 195. In conclusion, we indicated that DGCR5 could contribute to the radioresistance of human laryngeal carcinoma cells via sponging miR 195.
Pubmed ID	30549038
Year	2018
Title	Knockdown of DGCR5 enhances the radiosensitivity of human laryngeal carcinoma cells via inducing miR-195

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