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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	thyroid cancer
ICD-0-3	C73.9
Methods	qPCR, Luciferase reporter assay, etc.
Sample	PTC tissues, TPC1 PTC cell line and the Nthyori3-1 human thyroid epithelial cell line
Expression Pattern	down-regulated
Function Description	DGCR5 was significantly downregulated in PTC tissues compared with that in adjacent normal tissues. Through functional experiments, it was demonstrated that ectopic overexpression of DGCR5 markedly suppressed PTC cell growth and invasion. A bioinformatics analysis suggested that DGCR5 binds to microRNA (miR) 2861. A total of 5 putative binding sites for miR 2861 were identified in DGCR5, and a luciferase reporter assay confirmed the direct interaction between DGCR5 and miR 2861. Furthermore, reverse transcription quantitative polymerase chain reaction analysis indicated that ectopic overexpression of DGCR5 led to a decreased expression of miR 2861 in PTC cells and miR 2861 mimic transfection caused a downregulation of DGCR5. miR 2861 level was upregulated in PTC tissues compared with adjacent tissues and negatively correlated with DGCR5 level. In addition, rescue experiments indicated that ectopic expression of miR 2861 reversed the effects of DGCR5 overexpression on PTC cell proliferation and invasion. Taken together, the present results demonstrated that DGCR5 inhibits PTC progression via sponging miR 2861, indicating DGCR5 may serve as a therapeutic target.
Pubmed ID	30651878
Year	2019
Title	lncRNA DGCR5 acts as a tumor suppressor in papillary thyroid carcinoma via sequestering miR-2861

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