Basic Information
| LncRNA/CircRNA Name | DDN-AS1 |
| Synonyms | NA |
| Region | GRCh38_12:48998367-49019235 |
| Ensemble | ENSG00000257913 |
| Refseq | NR_147178 |
Classification Information
| Regulatory Mechanism | Biological Function | Clinical Application | |||
|---|---|---|---|---|---|
| TF | Immune | Survival | |||
| Enhancer | Apoptosis | Drug | |||
| Variant | Cell Growth | Circulating | |||
| MiRNA | EMT | Metastasis | |||
| Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
| Cancer Name | cervical cancer |
| ICD-0-3 | C53 |
| Methods | qPCR, Western blot, Luciferase reporter assay, etc. |
| Sample | Cancerous tissues, CC cell lines (ME180, HeLa, Caski, and SiHa) and one cervical immortalized cell line H8 |
| Expression Pattern | up-regulated |
| Function Description | Long noncoding RNAs (lncRNAs) are a group of regulators that have been widely reported in human malignant tumors including CC. On the basis of the data of The Cancer Genome Atlas, lncRNA DDN and PRKAG1 antisense RNA 1 (DDN AS1) that is overexpressed in CC tissues predicted poor prognosis for patients with CC. Moreover, quantitative reverse transcription PCR analysis further identified the upregulation of DDN AS1 in CC tissues and cell lines. Loss of function assays revealed that knockdown of DDN AS1 suppressed CC progression by efficiently inhibiting cell proliferation, migration, and invasion. Mechanism investigations revealed that DDN AS1 was upregulated by its upstream transcription activator transcription factor 3 (TCF3). Moreover, DDN AS1 increased the expression of TCF3 by competitively binding miR 15a and miR 16. In conclusion, DDN AS1 miR 15a/16 TCF3 feedback loop contributes to cell proliferation, migration, and invasion in CC. |
| Pubmed ID | 30582201 |
| Year | 2018 |
| Title | DDN-AS1-miR-15a/16-TCF3 feedback loop regulates tumor progression in cervical cancer. |
External Links
| Links for DDN-AS1 | GenBank HGNC NONCODE |
| Links for cervical cancer | OMIM COSMIC |