Basic Information
| LncRNA/CircRNA Name | circHIPK3 |
| Synonyms | |
| Region | |
| Ensemble | |
| Refseq |
Classification Information
| Regulatory Mechanism | Biological Function | Clinical Application | |||
|---|---|---|---|---|---|
| TF | Immune | Survival | |||
| Enhancer | Apoptosis | Drug | |||
| Variant | Cell Growth | Circulating | |||
| MiRNA | EMT | Metastasis | |||
| Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
| Cancer Name | liver cancer |
| ICD-0-3 | C22.0 |
| Methods | qRT-PCR, RNA FISH, Northern blotting, Oligonucleotide transfection, CCK-8 assay, EdU assay, RNA immunoprecipitation, Luciferase reporter assay |
| Sample | six normal tissues (brain, heart, liver, lung, colon and stomach), Human primary hepatocellular carcinoma and adjacent non-tumorous liver tissues, HEK-293 T, HeLa and HCT-116 cells |
| Expression Pattern | up-regulated |
| Function Description | Silencing of circHIPK3 inhibits human cell proliferation. we generate ribosomal-depleted RNA sequencing data from six normal tissues and seven cancers, and detect at least 27,000 circRNA candidates. Many of these circRNAs are differently expressed between the normal and cancerous tissues. We further characterize one abundant circRNA derived from Exon2 of the HIPK3 gene, termed circHIPK3. The silencing of circHIPK3 but not HIPK3 mRNA significantly inhibits human cell growth. Via a luciferase screening assay, circHIPK3 is observed to sponge to 9 miRNAs with 18 potential binding sites. Specifically, we show that circHIPK3 directly binds to miR-124 and inhibits miR-124 activity. Our results provide evidence that circular RNA produced from precursor mRNA may have a regulatory role in human cells. |
| Pubmed ID | 27050392 |
| Year | 2017 |
| Title | Circular RNA profiling reveals an abundant circHIPK3 that regulates cell growth by sponging multiple miRNAs |
External Links
| Links for circHIPK3 | GenBank HGNC NONCODE |
| Links for liver cancer | OMIM COSMIC |