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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	Breast Cancer
ICD-0-3	C50
Methods	qPCR, western blot, luciferase reporter assay, RIP, RNA-seq, other
Sample	breast cancer tissues and cell lines (MCF10A, T47D, MCF7, SK-BR-3, BT-474, MDA-MB-231, MDA-MB157, MDA-MB-436, HCC70, HCC1806, HCC1937, BT-549, and HEK293T)
Expression Pattern	up-regulated
Function Description	Gain-of-function and loss-of-function studies in BC cells demonstrated that circIRAK3 promoted cell migration, invasion and metastasis in vitro and in vivo but did not affect cell proliferation, colony formation or cell cycle progression. Using circIRAK3 in vivo precipitation and luciferase reporter assays, we identified miR-3607 as a circIRAK3-associated miRNA. Furthermore, RNA sequencing and bioinformatics analysis showed that forkhead box C1 (FOXC1), the target of miR-3607, was downregulated in circIRAK3-silenced cells and mediated circIRAK3-induced BC cell migration. Intriguingly, FOXC1 could, in turn, bind to the IRAK3 promoter, triggering a positive-feedback loop that perpetuated the circIRAK3/miR-3607/FOXC1 signaling axis.
Pubmed ID	29803789
Year	2018
Title	CircIRAK3 Sponges miR-3607 to Facilitate Breast Cancer Metastasis

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