Search Result

Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	lung adenocarcinoma
ICD-0-3	C34
Methods	qRT-PCR, CCK-8 cell proliferation assay, Cell apoptosis assay, Cell cycle analysis, Dual luciferase reporter assay, Western blotting
Sample	Lung adenocarcinoma tissues, Lung adenocarcinoma cell lines A549 and Calu-3 cells, normal human bronchial epithelial cells (NHBE) and HEK-293T (293T)
Expression Pattern	up-regulated
Function Description	hsa_circ_0025036 existed as a circular form and was aberrantly upregulated in lung adenocarcinoma tissues via qRT-PCR.?Its expression level exhibited a close link with aggressive clinicopathological parameters including cancer differentiation, TNM stage and lymph node metastasis.?hsa_circ_0025036 knockdown significantly suppressed cell proliferation and promoted cell apoptosis in A549 and Calu-3 cells.?Moreover, hsa_circ_0025036/miR-198/SHMT1&TGF-? axis was identified via bioinformatics analysis and dual-luciferase reporter assays.?miR-198 inhibitors reversed the function of hsa_circ_0025036 knockdown.?hsa_circ_0025036 knockdown exerted similar effects with miR-198 upregulation on cell proliferation and apoptosis.?In conclusion, we demonstrate that hsa_circ_0025036 regulates cell proliferation and apoptosis in lung adenocarcinoma cells probably via hsa_circ_0025036/miR-198/SHMT1&TGF-? axis.
Pubmed ID	30138108
Year	2018
Title	Aberrant expression of hsa_circ_0025036 in lung adenocarcinoma and its potential roles in regulating cell proliferation and apoptosis

External Links