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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	non small cell lung cancer
ICD-0-3	C34
Methods	qRT-PCR, Western blot, Northern blot with denaturing agarose gels, Luciferase reporter assay, RIP, RNA pull-down analysis, Fish
Sample	fresh NSCLC tissues, lung normal epithelial cell BEAS-2B, and NSCLC cells A549, H1299, H1650, SPC-A1, and Calu3 (lung adenocarcinoma cells) and H226, H520, and SK-MES-1 (lung squamous carcinoma cells)
Expression Pattern	down-regulated
Function Description	CircPTK2 and TIF1? were significantly down-regulated in NSCLC cells undergoing EMT induced by TGF-?. CircPTK2 overexpression augmented TIF1? expression, inhibited TGF-?-induced EMT and NSCLC cell invasion, whereas circPTK2 knockdown had the opposite effects. CircPTK2 functions as a sponge of miR-429/miR-200b3p, and miR-429/miR-200b-3p promote TGF-?-induced EMT and NSCLC cell invasion by targeting TIF1?. CircPTK2 overexpression inhibited the invasion-promoting phenotype of endogenous miR-429/miR-200b-3p in NSCLC cells in response to TGF-?. CircPTK2 overexpression significantly decreased the expression of Snail, an important downstream transcriptional activator of TGF-?/Smad signaling. In an in vivo experiment of metastasis, circPTK2 overexpression suppressed NSCLC cell metastasis. Moreover, circPTK2 expression was dramatically down-regulated and positively correlated with TIF1? expression in human NSCLC tissues. Especially, circPTK2 was significantly lower in metastatic NSCLC tissues than non-metastatic counterparts.
Pubmed ID	30261900
Year	2018
Title	Circular RNA hsa_circ_0008305 (circPTK2) inhibits TGF-?-induced epithelialmesenchymal transition and metastasis by controlling TIF1? in non-small cell lung cancer

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